Dr Jonathan Kyffin
Lecturer in Biology
Jonathan is a current academic member of the School of Natural Sciences focussing on the provision of teaching for Biological Sciences students.
Jonathan currently teaches Cell Biology and Biochemistry, and Introductory Microbiology and Immunology at level 4 for a range of undergraduate degree courses. At level 5 he is involved in the teaching of Biology of Animal disease, Research Methods and Microbiology for Animal Sciences. Additionally, he leads Forensic Toxicology at level 6 for toxicology and pathology students. Jonathan also spends time supervising students involved in laboratory based dissertation projects for final year students.
Jonathan's main research interest is focused on the development and application of novel in vitro 3D models for toxicological investigations in parallel with in silico modelling and optimization. His work aims to bridge the complexity gap between classic 2D cellular platforms and more complex 3D and bioreactor systems in order to provide sufficient toxicological information for early decision making during the research of novel xenobiotics.
Jonathan has other research interests including the evaluation of regulatory OECD guidelines and the establishment of novel modelling approaches for developmental and reproductive toxicology (DART). He is currently collaboratively working with colleagues from Liverpool John Moores University, the University of Liverpool and Syngenta Ltd.
Multiscale modelling of drug transport and metabolism in liver spheroids
6 Apr 2020 Interface Focus 10(2):20190041 The Royal Society
Co-authors: Leedale JA, Kyffin JA, Harding AL, Colley HE, Murdoch C, Sharma P, Williams DP, Webb SD, Bearon RN
10.1098/rsfs.2019.0041
Kyffin, J.A., et al., Impact of cell types and culture methods on the functionality of in vitro liver systems – A review of cell systems for hepatotoxicity assessment. Toxicology in Vitro, 2018. 48C: p. 262-275.
Kyffin, J.A., et al., Characterisation of a functional rat hepatocyte spheroid model. Toxicology in Vitro, 2019. 55: p. 160-172.
Tomlinson, L., et al., In vitro liver zonation of primary rat hepatocytes. Front. Bioeng. Biotechnol. 7:17.
Kyffin, J. A., Cox, C. R., Leedale, J., Colley, H. E., Murdoch, C., Mistry, P., Webb, S. D., & Sharma, P. (2019). Preparation of primary rat hepatocyte spheroids utilizing the liquid-overlay technique. Current Protocols in Toxicology, e87.
The Toxicologist, Supplement to Toxicological Sciences, 150 (1), Abstract #1260, 2017, Optimization of primary rat hepatocyte spheroids via computational modelling as a platform for toxicological investigations, J. A. Kyffin.
Optimisation of species-specific 3D liver microtissues via computational modelling for repeat-dose toxicology. Poster abstract presentation QSP Surrey 2016, J. A. Kyffin.
Establishing species-specific 3D liver microtissues for repeat-dose toxicology and advancing in vitroto in vivo translation through computational modelling. Poster abstract presentation IVTS 2015, J. A. Kyffin.